An emerging lineage of uropathogenic extended spectrum beta-lactamase Escherichia coli ST127

Cave, Rory, Ter-Stepanyan, Mary M, Kotsinyan, Nune and Mkrtchyan, Hermine (2022) An emerging lineage of uropathogenic extended spectrum beta-lactamase Escherichia coli ST127. Microbiology Spectrum. (In Press)

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Abstract

Uropathogenic Escherichia coli (UPEC) is one of the most common causes of urinary tract infections. Here we report for the first time the whole genome sequencing (WGS) and analysis of four extended-spectrum beta-lactamase (ESBL) UPEC sequence type (ST) 127 isolates recovered from patients in five hospitals in Armenia between January to August 2019. Phylogenetic comparison revealed that our isolates were closely related to each other by the core and accessory genomes despite being isolated from different regions and hospitals in Armenia. We identified unique genes in our isolates and in a closely related isolate recovered in France. The unique genes (hemolysin E virulence gene, lactate utilisation operon lutABC and endonuclease restriction modification operon hsdMSR) were identified in three separate genomic regions adjacent to prophage genes, including one region containing the TonB-dependent iron siderophore receptor gene ireA, which was only found in 5 other ST127 isolates from the European Nucleotide Archive (ENA). We further identified that these isolates possessed unique virulence and metabolic genes and harboured antibiotic resistance genes, including ESBL genes blaCTX-M-3 (n=3), blaCTX-M-236 (n=1) and blaTEM-1 (n=1), in addition to a quinolone resistance protein gene qnrD1 (n=1), which was absent in ST127 isolates obtained from ENA. Moreover, a plasmid replicon gene IncI2 (n=1) was unique to ARM88 of the Armenian isolates. Our findings demonstrate that at the time of this study E. coli ST127 was a cause of urinary tract infections in patients in different regions of Armenia, with a possibility of cross-country transmission between Armenia and France.

Item Type: Article
Subjects: Medicine and health > Microbiology
Related URLs:
Depositing User: Hermine Mkrtchyan
Date Deposited: 09 Nov 2022 13:59
Last Modified: 10 Nov 2022 09:24
URI: https://repository.uwl.ac.uk/id/eprint/9605

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