Influence of soy fortification on microbial diversity during cassava fermentation and subsequent physicochemical characteristics of garri

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Ahaotu, Ndidiamaka Nnenaya, Anyogu, Amarachukwu ORCID: https://orcid.org/0000-0001-9652-7728, Obioha, Promiselynda, Aririatu, Lawrence, Ibekwe, Vincent Ifeanyi, Oranusi, Solomon, Sutherland, Jane P. and Ouoba, Labia Irene Ivette (2017) Influence of soy fortification on microbial diversity during cassava fermentation and subsequent physicochemical characteristics of garri. Food Microbiology, 66. pp. 165-172. ISSN 0956-7135

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Official URL: https://doi.org/10.1016/j.fm.2017.04.019

Abstract

This study investigated the influence of the addition of soy products on the microbiology, nutritional and physico-chemical characteristics of garri, a fermented cassava product. Malted soy flour (MSF) and soy protein (SP) were separately added (12% w/w) to cassava mash prior to controlled fermentation, while non-supplemented cassava mash served as a control. Identification of lactic acid bacteria (LAB) and aerobic mesophilic bacteria was accomplished by repetitive sequence-based (rep)-PCR analysis and 16S rRNA gene sequencing.

Physicochemical, nutritional and sensory characterisation of control and soy-fortified garri was performed using conventional methods. rep-PCR allowed differentiation of 142 isolates into 41 groups corresponding to 6 species of LAB and 25 species of aerobic mesophiles. LAB isolates belonged to the genera Lactobacillus, Weissella, Leuconostoc and Lactococcus with Leuconostoc mesenteroides being the dominant species in control and MSF-cassava while Weissella cibaria dominated SP-cassava fermentation. Aerobic mesophiles included Gram positive and negative bacteria such species of the genera Bacillus, Clostridium, Staphylococcus, Serratia, Acinetobacter and Raoultella. Diversity of aerobic mesophiles varied between control, MSF- and SP- cassava mash. Protein content of soy-fortified garri increased from 0.73% to 10.17% and 10.05% in MSF and SP garri respectively with a significant decrease in total cyanide from 26 to 11 ppm.

Results from physicochemical and organoleptic evaluation indicate that supplementation of cassava with soy products prior to fermentation can produce acceptable garri. Soy products can be considered a viable option for protein fortification of garri, a low protein food with the aim of combating malnutrition.

Item Type:	Article
Identifier:	10.1016/j.fm.2017.04.019
Additional Information:	The authors acknowledge the support of the Federal University of Technology, Owerri for funding this research through the Tertiary Education Trust Fund (TETFund) intervention programme.
Keywords:	garri; cassava; soy products; fortification; lactic acid bacteria; aerobic mesophiles
Subjects:	Medicine and health > Microbiology
Related URLs:	Publisher
Depositing User:	Amarachukwu Anyogu
Date Deposited:	10 Sep 2021 10:10
Last Modified:	06 Feb 2024 16:06
URI:	https://repository.uwl.ac.uk/id/eprint/8229

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Influence of soy fortification on microbial diversity during cassava fermentation and subsequent physicochemical characteristics of garri

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