Identification of novel antioxidant peptides from snakehead (Channa argus) soup generated during gastrointestinal digestion and insights into the anti-oxidation mechanisms

Zhang, J, Li, M, Zhang, G, Tian, Y, Kong, F, Xiong, S, Zhao, S, Jia, D, Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722 and Du, H (2021) Identification of novel antioxidant peptides from snakehead (Channa argus) soup generated during gastrointestinal digestion and insights into the anti-oxidation mechanisms. Food Chemistry, 337. ISSN 0308-8146

[img] PDF
Zhang et al (2021) Identification of novel antioxidant peptides.pdf - Accepted Version
Restricted to Repository staff only until 28 August 2021.
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (1MB)

Abstract

Antioxidant peptides obtained from snakehead (Channa argus) soup (SHS) after simulated gastrointestinal (GI) digestion were separated, identified and characterized. Results showed that the fraction with MW < 3 kDa had the highest antioxidant capacity. Four novel antioxidant peptides were identified after RP-HPLC and UPLC-MS/MS. PGMLGGSPPGLLGGSPP and SDGSNIHFPN had the highest DPPH radical scavenging activity (IC50 = 1.39 mM) and Fe2+ chelating ability (IC50 = 4.60 mM), respectively. Structures in silico for IVLPDEGK, PGMLGGSPPGLLGGSPP and SDGSNIHFPN suggest at least one β-turn and/or α-helix, which are associated with antioxidant activity. Moreover, our results showed that these three peptides docked with a recombinant Kelch-like ECH-associated protein 1 (Keap1) with a binding score greater than TX6, a good ligand of Keap1. The cell viability assay also showed significant cytoprotective effects against H2O2-induced cellular oxidative damage. This information implies that antioxidant mechanisms of novel SHS peptides occurred via activation of cellular anti-oxidation Keap1-Nrf2 signaling pathway.

Item Type: Article
Uncontrolled Keywords: Antioxidant peptides, Snakehead soup, Gastrointestinal digestion, Molecular docking, UPLC-MS/MS, RP-HPLC, Kelch-like ECH-associated protein 1, Cytoprotective effect
Subjects: Natural sciences > Cell and molecular biology
Medicine and health > Nutrition
Depositing User: Anne Manyande
Date Deposited: 30 Nov 2020 18:17
Last Modified: 16 Feb 2021 17:29
URI: http://repository.uwl.ac.uk/id/eprint/7500

Actions (login required)

View Item View Item

Menu