Identification of novel antioxidant peptides from snakehead (Channa argus) soup generated during gastrointestinal digestion and insights into the anti-oxidation mechanisms

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Zhang, J, Li, M, Zhang, G, Tian, Y, Kong, F, Xiong, S, Zhao, S, Jia, D, Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722 and Du, H (2021) Identification of novel antioxidant peptides from snakehead (Channa argus) soup generated during gastrointestinal digestion and insights into the anti-oxidation mechanisms. Food Chemistry, 337. ISSN 0308-8146

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Official URL: https://doi.org/10.1016/j.foodchem.2020.127921

Abstract

Antioxidant peptides obtained from snakehead (Channa argus) soup (SHS) after simulated gastrointestinal (GI) digestion were separated, identified and characterized. Results showed that the fraction with MW < 3 kDa had the highest antioxidant capacity. Four novel antioxidant peptides were identified after RP-HPLC and UPLC-MS/MS. PGMLGGSPPGLLGGSPP and SDGSNIHFPN had the highest DPPH radical scavenging activity (IC50 = 1.39 mM) and Fe2+ chelating ability (IC50 = 4.60 mM), respectively. Structures in silico for IVLPDEGK, PGMLGGSPPGLLGGSPP and SDGSNIHFPN suggest at least one β-turn and/or α-helix, which are associated with antioxidant activity. Moreover, our results showed that these three peptides docked with a recombinant Kelch-like ECH-associated protein 1 (Keap1) with a binding score greater than TX6, a good ligand of Keap1. The cell viability assay also showed significant cytoprotective effects against H2O2-induced cellular oxidative damage. This information implies that antioxidant mechanisms of novel SHS peptides occurred via activation of cellular anti-oxidation Keap1-Nrf2 signaling pathway.

Item Type:	Article
Identifier:	10.1016/j.foodchem.2020.127921
Keywords:	Antioxidant peptides, Snakehead soup, Gastrointestinal digestion, Molecular docking, UPLC-MS/MS, RP-HPLC, Kelch-like ECH-associated protein 1, Cytoprotective effect
Subjects:	Natural sciences > Cell and molecular biology Medicine and health > Nutrition
Depositing User:	Anne Manyande
Date Deposited:	30 Nov 2020 18:17
Last Modified:	04 Nov 2024 11:46
URI:	https://repository.uwl.ac.uk/id/eprint/7500