Conjugative transfer frequencies of mef(A)-containing Tn1207.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types

Hadjirin, Nazreen F., Harrison, E.M., Holmes, M.A. and Paterson, G.K. (2014) Conjugative transfer frequencies of mef(A)-containing Tn1207.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types. Letters in Applied Microbiology, 58 (4). pp. 299-302. ISSN 0266-8254

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Abstract

The aim of this study was to examine the gene transfer potential of mef(A)-containing Tn120.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types. Using the filter mating technique, Tn1207.3 was transferred by conjugation to 23 macrolide-susceptible recipients representing 11 emm types. PCR analysis confirmed the presence of the mef(A) gene and the comEC junction regions of the Tn1207.3 insertion in resultant transconjugants. Significant variation was found in the transfer frequency of Tn1207.3 to different Strep. pyogenes strains, and this phenomenon may contribute to the differences in mef(A) frequency observed among clinical isolates.

Item Type: Article
Additional Information: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords: antibiotics; molecular epidemiology; streptococci; transposons
Subjects: Medicine and health > Microbiology
Depositing User: Dominic Walker
Date Deposited: 13 Jan 2017 11:31
Last Modified: 23 Jun 2017 10:40
URI: http://repository.uwl.ac.uk/id/eprint/3027

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