Xu, Zhen, Misra, Raju, Jamrozy, Dorota, Paterson, Gavin K., Cutler, Ronald R., Holmes, Mark A., Gharbia, Saheer and Mkrtchyan, Hermine (2018) Whole genome sequence and comparative genomics analysis of multi-drug resistant environmental Staphylococcus epidermidis ST59. G3: Genes|Genomes|Genetics, 8 (7). pp. 2225-2230. ISSN 2160-1836

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Abstract

Staphylococcus epidermidis is a major opportunistic pathogen primarily recovered from device-associated healthcare associated infections (DA-HAIs). Although S. epidermidis and other coagulase-negative staphylococci (CoNS) are less virulent than Staphylococcus aureus, these bacteria are an important reservoir of antimicrobial resistance genes and resistance-associated mobile genetic elements that can be transferred between staphylococcal species. We report a whole genome sequence of a multidrug resistant S. epidermidis (strain G6_2) representing multilocus sequence type (ST) 59 and isolated from an environmental sampling of a hotel room in London, UK. The genome of S. epidermidis G6_2 comprises of a 2408357 bp chromosome and six plasmids, with an average G+C content of 32%. The strain displayed a multi-drug resistance phenotype which was associated with carriage of 7 antibiotic resistance genes (blaZ, mecA, msrA, mphC, fosB, aacA-aphD, tetK) as well as resistance-conferring mutations in fusA and ileS. Antibiotic resistance genes were located on plasmids and chromosome. Comparative genomic analysis revealed that antibiotic resistance gene composition found in G6_2 was partly preserved across the ST59 lineage.

Item Type:	Article
Identifier:	10.1534/g3.118.200314
Additional Information:	Copyright © 2018 Xuet al. Supplemental material available at Figshare: https://doi.org/10.25387/g3.6133946
Keywords:	Staphylococcus epidermidis, antibiotic resistance, whole genome sequence, comparative analysis
Subjects:	Medicine and health > Health promotion and public health > Infection prevention Medicine and health
Related URLs:	https://doi.org/10.25387/g3.6133946
Depositing User:	Jisc Router
Date Deposited:	16 Sep 2020 15:11
Last Modified:	04 Nov 2024 11:47
URI:	https://repository.uwl.ac.uk/id/eprint/7310

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