Manjuck, J., Saha, D. C., Astiz, Mark E., Eales-Reynolds, Lesley-Jane ORCID: https://orcid.org/0000-0002-9906-2484 and Rackow, E. C. (2000) Decreased response to recall antigens is associated with depressed costimulatory receptor expression in septic critically ill patients. Journal of Laboratory and Clinical Medicine, 135 (2). pp. 153-160. ISSN 0022-2143

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Abstract

Anti-inflammatory substances are released during septic shock that modulate monocyte function. Decreased monocyte responsiveness to bacterial toxins and decreased expression of human-leukocyte-associated antigen-DR (HLA-DR) have been reported during septic shock and critical illness. Impaired antigen presentation has been inferred from these observations but has not been demonstrated. We assessed antigen presentation and costimulatory molecule expression in 12 age-matched control subjects, 10 noninfected critically ill patients (CINS), and 17 critically ill patients with sepsis (CIS). Antigen presentation was assessed by using in vitro lymphocyte 5-bromo-2-deoxyuridine (BrdU) incorporation in response to tetanus toxoid. The expression of HLA-DR and the costimulatory molecules CD28, CD86, and CTLA-4 was assessed by flow cytometry. Serum interleukin-10 (IL-10) was also measured by enzyme-linked immunosorbent assay. Serum IL-10 levels were significantly elevated in CIS patients (91 +/- 38 pg/mL) as compared with levels in control subjects (5 +/- 4 pg/mL)(P \textless .05). Lymphocyte BrdU incorporation increased by 710% +/- 243% in control subjects but by only 144% +/- 62% in CIS patients and 76% +/- 31% in CINS patients (P \textless .01 vs control). Monocyte HLA-DR expression, monocyte CD86 expression, and lymphocyte CD28 expression were significantly decreased in CIS patients (P \textless .01) as compared with control subjects. Conversely, lymphocyte CTLA-4 expression was significantly increased in CIS patients (P \textless .05 vs control). Monocyte CD86 expression was also significantly decreased in CINS patients as compared with control subjects. These data indicate that antigen presentation is decreased in critically ill patients with sepsis. This appears in part related to decreased expression of HLA-DR and the costimulatory molecules CD86 and CD28. Increased expression of the negative signal receptor CTLA-4 may also impair antigen presentation in patients with sepsis.

Item Type:	Article
Keywords:	Lymphocytes, Male, Humans, Lymphocyte Activation, HLA-DR Antigens, Monocytes, Cells, Cultured, Female, Middle Aged, Antigens, CD, Antigens, CD28, Antigens, CD86, Antigens, Differentiation, Bacterial Toxins, Critical Illness, CTLA-4 Antigen, Immunoconjugates, Interleukin-10, Membrane Glycoproteins, Reference Values, Shock, Septic
Subjects:	Medicine and health > Clinical medicine Medicine and health
Depositing User:	Lesley-Jane Eales-Reynolds
Date Deposited:	20 May 2018 08:15
Last Modified:	06 Feb 2024 15:57
URI:	https://repository.uwl.ac.uk/id/eprint/5058

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