Jason, Tallis, Caitlin, Mcmorrow, Sharn, Shelley and Steven, Eustace (2025) Effects of high-fat diet and nutraceutical resveratrol on maximal force, power and fatigue resistance of skeletal muscle. Journal of Pharmacological and Toxicological Methods, 133. ISSN 1056-8719

Full text not available from this repository.

Abstract

Benefits of utilizing models of disease in order to facilitate and improve the drug discovery and development process is becoming increasingly recognized. Certain drug classes (ie Tyrosine Kinase Inhibitors and HMG-Co inhibitors) have been shown to induce skeletal muscle dysfunction as a side effect. Assessment of drug effects on skeletal muscle performance early in drug development process is important in understanding unplanned drug effects; healthy/disease models assessing skeletal muscle function, which replicate In vivo function, are limited. The objective of the present study was to compare the contractile properties of isolated soleus (slow-twitch) and extensor digitorum longus (EDL; fast-twitch) muscle in control and high-fat diet (HFD) conditions, in addition to effects of nutraceutical resveratrol. Four-week-old female mice (CD1; N = 40 starting sample; N = 38 final sample) were randomly assigned to control (SLD) or HFD, with (SLD RES, HFD RES) or without resveratrol (4 g.kg−1 diet) for 12-weeks. Soleus and EDL (N = 8–10) were isolated and absolute and normalized force and power output (PO) were measured, and measures of fatigue were determined. For soleus, absolute force was significantly greater in HFD RES Vs. HFD only (P = 0.030), and PO normalized to body mass was diminished in HFD treated groups (P < 0.001); no other differences were observed (P > 0.584). For EDL, absolute and normalized force did not differ between groups (P > 0.091). The HFD induced reduction in absolute and normalized EDL PO, and cumulative work production (P < 0.019) was attenuated with the nutraceutical resveratrol, with HFD RES producing comparable PO and work as SLD groups (P > 0.101). Our data suggests that high fat diet conditions cause a decline in soleus and EDL skeletal muscle function. Despite the nutraceutical resveratrol having little effect on the contractile properties in SLD groups it did attenuate the decline in fast-twitch HFD skeletal muscle function. This data indicates that nutraceutical resveratrol may be effective in reducing the impact of HFD on skeletal muscle function. Furthermore, this data highlights that drug induced changes in skeletal muscle function should be explored in both healthy and disease models across multiple muscle phenotypes and modes of contractility, as drug induced changes are not uniform across populations, muscles or contractile modes.

Item Type:	Article
Identifier:	10.1016/j.vascn.2025.107675
Subjects:	Medicine and health
Depositing User:	Steven Eustace
Date Deposited:	02 Sep 2025 13:34
Last Modified:	02 Sep 2025 13:34
URI:	https://repository.uwl.ac.uk/id/eprint/14003

Actions (login required)

View Item