Prevalence, persistence and diversity of Listeria strains with antimicrobial

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Areo, Esther Omobola, Anyogu, Amarachukwu ORCID: https://orcid.org/0000-0001-9652-7728, Fowora, Muinah A., Omemu, Mobolaji O., Oyewole, Olusola B. and Obadina, Adewale Olusegun (2025) Prevalence, persistence and diversity of Listeria strains with antimicrobial. The Microbe, 7. pp. 1-17.

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Abstract

Untreatable listeriosis and wastage could be traced to contaminated fruits. This study assessed Listeria spp, antimicrobial resistance and virulence genes in ready-for-sale fruits. Listeria spp was identified in 270 fruits: garden egg (90), tomato (90) and watermelon (90), were purchased from thirty markets, in Southwest Nigeria. Listeria spp were evaluated, identified and sequenced. Antimicrobial sensitivity assay (15 antibiotics), eighteen antimicrobial and nine virulence genes were screened for. Listeria spp 28 (100.00 %) at 66.25 MPN/g comprising of pathogenic (19) (L. monocytogenes 6 (21.43 %), L. ivanovii 5 (17.86 %), L. seegligeri 8 (28.57 %)) and non-pathogenic (9) (L. welshimeri 5 (17.86 %), L. grayi 2 (7.14 %) and L. innocua 1 (3.57 %)) strains were distributed in garden egg 8 (28.57 % at 56.63 MPN/g), tomato 14 (50.00 % at 54.29 MPN/g) and watermelon 6 (21.43 % at 57.00 MPN/g). Carbapenem, chloramphenicol, macrolides, tetracycline and folate resistant Listeria strains with highest prevalence were in fruit from Balogun 9 (60.00 %), Agege 8 (28.57 %) and Lekki 10 (45.45 %) in Lagos state. Virulent L. strains had five L. monocytogenes, two L. ivanovii, eight L. seegligeri, five L. welshimeri, two L. innocua and eight L. grayi in fruit from Lagos, Osun and Ondo States respectively. Listeria monocytogenes, L. ivanovii and L. seegligeri in fruit from Lagos State had prfA, plcA and plcB genes. Fruits could thereby be versatile route for various diverse virulent-resistant Listeria strains that could cause constant listeriosis and spoilage. However, there is need for more enforced, good and healthy handling of fruits on farms and in markets.

Item Type:	Article
Identifier:	10.1016/j.microb.2025.100286
Keywords:	Listeria monocytogenes, Pathogenic, Listeria strains, Non-pathogenic, Listeria strains, Virulence genes, Antimicrobial resistance genes, Fresh fruits
Subjects:	Medicine and health > Microbiology Natural sciences > Cell and molecular biology
Depositing User:	Amara Anyogu
Date Deposited:	25 Apr 2025 08:13
Last Modified:	25 Apr 2025 08:15
URI:	https://repository.uwl.ac.uk/id/eprint/13491

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Prevalence, persistence and diversity of Listeria strains with antimicrobial

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