Analysis of the binding selectivity and inhibiting mechanism of chlorogenic acid isomers and their interaction with grass carp endogenous lipase using multi-spectroscopic, inhibition kinetics and modeling methods

Xu, Zeru, Du, Hongying, Cao, Qiongju, Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722 and Xiong, Shanbai (2022) Analysis of the binding selectivity and inhibiting mechanism of chlorogenic acid isomers and their interaction with grass carp endogenous lipase using multi-spectroscopic, inhibition kinetics and modeling methods. Food Chemistry, 382. p. 132106. ISSN 0308-8146

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Abstract

Polyphenols are inhibitors for lipase, but the binding selectivity and mechanism of polyphenol isomers and how they interact with lipase are not clear. Here, chlorogenic acid (CGA) isomers, neochlorogenic acid (NCGA) and cryptochlorogenic acid (CCGA) were used to explore the binding selectivity and mechanism of lipase. An inhibition assay indicated that both CGA isomers had dose-dependent inhibitory effects on lipase; however, the inhibitory effect of NCGA was better (IC50: 0.647 mg/mL) than that of CCGA (IC50: 0.677 mg/mL). NCGA and CCGA formed complexes with lipase at a molar ratio of 1:1, and the electrostatic interaction force plays a major role in the lipase–CCGA system. Molecular dynamics studies demonstrated that NCGA had a greater impact on the structure of lipase. The multi-spectroscopic and modeling results explained the effects of micro-structural changes on the binding site, the interaction force and the inhibition rate of the isomers when they combined with lipase.

Item Type: Article
Identifier: 10.1016/j.foodchem.2022.132106
Additional Information: First available online 10 January 2022
Keywords: Lipase, Neochlorogenic acid, Cryptochlorogenic acid, Interaction mechanism, Molecular dynamics simulation
Subjects: Medicine and health > Nutrition
Related URLs:
Depositing User: Anne Manyande
Date Deposited: 04 Feb 2022 12:37
Last Modified: 18 Aug 2022 13:39
URI: https://repository.uwl.ac.uk/id/eprint/8769

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