Comprehensive analysis of transcriptomics and metabolomics to understand the flesh quality regulation of crucian carp (Carassius auratus) treated with short term micro-flowing water system

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Du, H, Xiong, S, Lv, H, Zhao, S and Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722 (2021) Comprehensive analysis of transcriptomics and metabolomics to understand the flesh quality regulation of crucian carp (Carassius auratus) treated with short term micro-flowing water system. Food Research International, 147. p. 110519. ISSN 0963-9969

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Du et al (2021). Comprehensive analysis of transcriptomics and metabolomics to understand the flesh quality regulation of crucian carp (Carassius auratus) treated with short term micro-flowing water system.pdf - Accepted Version
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Official URL: https://doi.org/10.1016/j.foodres.2021.110519

Abstract

The short term micro-flowing purification system (STMFPS) has been shown to improve the flesh quality of freshwater fish. However, few studies have focused on the involved underlying mechanisms. This study explored the effect of STMFPS on the flesh quality of market-size freshwater fish based on the combination of metabolomics and transcriptomics methods. The UPLC-QTOF/MS based metabolomics method was utilized to screen metabolites and predict the possible major metabolic pathways during different STMFPS treatment periods (0 d, 1 d, 5 d and 9 d). Furthermore, the transcriptomic data demonstrated that the deferentially expressed genes detected in crucian carp muscle were 2915,7852 and 7183 after 1 d, 5 d and 9 d STMFPS treatment. Results showed that the TCA cycle, ornithine cycle, purine metabolism and amino acid catabolism play important roles in improving the flesh quality of crucian carp. This study may help to understand the mechanism of improving the flesh quality of aquatic products using STMFPS.

Item Type:	Article
Identifier:	10.1016/j.foodres.2021.110519
Keywords:	Freshwater fish, Mass spectrometry (MS); Genes; Metabolites; Principal component analysis (PCA); Orthogonal partial least squares discriminant analysis (OPLS-DA)
Subjects:	Medicine and health Medicine and health > Nutrition
Related URLs:	Publisher
Depositing User:	Anne Manyande
Date Deposited:	15 Jun 2021 11:14
Last Modified:	06 Feb 2024 16:05
URI:	https://repository.uwl.ac.uk/id/eprint/7957