Qualitative and quantitative analysis of regional cerebral free fatty acids in rats using the stable isotope labeling liquid chromatography–mass spectrometry method

Hu, T, Zhu, Q, Hu, Y, Kamal, GM, Feng, Y, Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722, Wang, J and Xu, F (2020) Qualitative and quantitative analysis of regional cerebral free fatty acids in rats using the stable isotope labeling liquid chromatography–mass spectrometry method. Molecules, 25 (21). p. 5163.

[img]
Preview
PDF
Hu et al (2021) Qualitative and Quantitative Analysis of Regional Cerebral Free Fatty Acids .pdf - Accepted Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview
[img]
Preview
PDF
molecules-25-05163.pdf - Published Version
Available under License Creative Commons Attribution.

Download (3MB) | Preview

Abstract

Free fatty acids serve as important bioactive molecules in the brain. They are involved in message transfer in the brain. There are many reports available in the literature regarding the role of cerebral fatty acids in message transfer; however, most of the studies are mainly focused on limited fatty acid species or only a few specific brain regions. To understand the relationship between cerebral functions and free fatty acids, it is necessary to investigate the distribution of the free fatty acids among different regions in the whole brain. In this study, free fatty acids were extracted from different brain regions and analyzed qualitatively and quantitatively using the stable isotopic labeling liquid chromatography–mass spectrometry approach. In total, 1008 potential free fatty acids were detected in the whole brain out of which 38 were found to be commonly present in all brain regions. Among different brain regions, the highest and the smallest amounts of potential free fatty acids were detected in the olfactory bulb and cerebellum, respectively. From a statistical point of view, 4-methyl-2-oxovaleric acid, cis-11, 14-eicosadienoic acid, tridecanoic acid, myristic acid, nonadecanoic acid, and arachidic acid were found to significantly vary among the four different brain regions (olfactory bulb, occipital lobe, hippocampus, and cerebellum). The variation in the composition of free fatty acids among different brain regions may be very important for investigating the relationship between free fatty acids and functions of cerebral regions.

Item Type: Article
Uncontrolled Keywords: fatty acids; brain; liquid chromatography–mass spectrometry method; stable isotope labeling; regions
Subjects: Medicine and health > Research methodologies
Related URLs:
Depositing User: Anne Manyande
Date Deposited: 30 Nov 2020 19:02
Last Modified: 30 Nov 2020 22:26
URI: http://repository.uwl.ac.uk/id/eprint/7502

Downloads

Downloads per month over past year

Actions (login required)

View Item View Item

Menu