Molecular characterization of methicillin-resistant and -susceptible Staphylococcus aureus recovered from hospital personnel

Xu, Zhen, Li, Xiaodong, Tian, Dan, Sun, Zhuoyu, Guo, Liqiong, Dong, Cuixia, Tang, Naijun and Mkrtchyan, Hermine (2020) Molecular characterization of methicillin-resistant and -susceptible Staphylococcus aureus recovered from hospital personnel. Journal of Medical Microbiology. ISSN 0022-2615

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Abstract

Introduction
Methicillin resistant Staphylococcus aureus (MRSA) is one of the major causes of hospital acquired infections. Over the past two decades MRSA has become ‘epidemic’ in many hospitals worldwide. However, little is known about the genetic background of S. aureus recovered from hospital personnel in China.
Aim
The aim of this study was to determine the genetic diversity of MRSA and methicillin susceptible S. aureus (MSSA) recovered from hospital personnel in Tianjin, North China.
Methodology
Three hundred and sixty-eight hand or nasal swabs were collected from 276 hospital personnel in four tertiary hospitals in Tianjin, North China between November 2017 and March 2019. In total, 535 gram-positive bacteria were isolated, of which 59 were identified as S. aureus. Staphylococcal cassette chromosome mec (SCCmec) typing, multi-locus sequence typing (MLST) and spa typing were performed to determine molecular characteristics of S. aureus.
Results
Thirty-one out of 276 (11%) hospital personnel were S. aureus carriers, whereas 11/276 (4%) carried MRSA. Fifty out of 59 (85%) of S. aureus isolates were resistant or intermediate resistant to erythromycin. The dominant genotypes of MRSA recovered from hospital personnel were ST398-t034-SCCmecIV/V, and ST630-t084/t2196, whereas major genotypes of MSSA included ST15-t078/t084/t346/t796/t8862/ t8945/t11653 and ST398-t189/t034/ t078/t084/t14014.
Conclusion
Although, the predominant genotypes of MRSA recovered from hospital personnel in this study were different from those main genotypes that have previously been reported to cause infections in Tianjin and in other geographic areas of China, the MRSA ST398-t034 genotype has previously been reported to be associated with livestock globally. The dominant MSSA genotypes recovered from hospital personnel were consistent with those previously reported MSSA genotypes recovered from the clinic. The diversity of S. aureus genotypes warrantee further surveillance and genomic studies to better understand the relatedness of these bacteria with those recovered from patients and community.

Item Type: Article
Additional Information: Zhen Xu et al., J Med Microbiol, 2020. © The Authors, 2020. The definitive peer reviewed, edited version of this article is published in Journal of Medical Microbiology, 2020, https://doi.org/10.1099/jmm.0.001272.
Uncontrolled Keywords: MLST, spa typing, hospital personnel and Staphylococcus aureus
Subjects: Medicine and health > Microbiology
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Depositing User: Jisc Router
Date Deposited: 16 Oct 2020 19:06
Last Modified: 17 Nov 2020 10:11
URI: http://repository.uwl.ac.uk/id/eprint/7402

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