Antibiotic resistance and mecA characterization of coagulase-negative staphylococci isolated from three hotels in London, UK

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Xu, Zhen, Mkrtchyan, Hermine and Cutler, Ronald R. (2015) Antibiotic resistance and mecA characterization of coagulase-negative staphylococci isolated from three hotels in London, UK. Frontiers in Microbiology, 6. ISSN 1664-302X

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Official URL: https://doi.org/10.3389/fmicb.2015.00947

Abstract

Antibiotic resistance in bacteria isolated from non-healthcare environments, is a potential problem to public health. In our survey a total of 71 coagulase negative staphylococci (CNS) belonging to 11 different species were isolated from three large hotels in London, UK. The most prevalent species was Staphylococcus haemolyticus, with S. hominis, S. warneri, S. cohnii, and Staphylococcus epidermidis commonly detected. Antimicrobial susceptibilities and carriage of the mecA gene were determined for all of these isolates. Most (85.9%) staphylococci were resistant to multiple antibiotics with all displaying increased susceptibility toward penicillin, fusidic acid, erythromycin, and cefepime. Twenty-one (29.5%) of the isolates were mecA positive, however MIC values to oxacillin, normally associated with the carriage of mecA, varied widely in this group (from 0.06 to 256 mg/L). Fifteen of the twenty-one mecA positive isolates carried SCCmec of these seven were type V, one type I, one type II, and one type IV. Additionally, five of these 15 isolates carried a previously unreported type, 1A, which involves an association between class A mec complex and ccr type 1. The remaining six of the 21 isolates were non-typeable and carried a combination of class A mec complex and ccrC. In addition to this, we also report on new MLST types which were assigned for five S. epidermidis isolates. Four out of these five isolates had MICs between 0.06 and 256mg/L to oxacillin and would be regarded as clinically susceptible but one isolate had a high oxacillin MIC of 256 mg/L. We demonstrated widespread multiple drug resistance among different staphylococcal species isolated from non-healthcare environments highlighting the potential for these species to act as a reservoir for methicillin and other forms of drug resistance.

Item Type:	Article
Identifier:	10.3389/fmicb.2015.00947
Additional Information:	Copyright © 2015 Xu, Mkrtchyan and Cutler. This is the final version of an article (Citation: Xu Z, Mkrtchyan HV and Cutler RR (2015) Antibiotic resistance and mecA characterization of coagulase-negative staphylococci isolated from three hotels in London, UK. Front. Microbiol. 6:947. doi: 10.3389/fmicb.2015.00947) which first appeared in Fontiers in Microbiology published by Frontiers Media on 9 September 2015.
Keywords:	antibiotic resistance, coagulase-negative staphylococci, mecA gene, SCCmec typing, MLST
Subjects:	Medicine and health > Microbiology Medicine and health
Related URLs:	Publisher
Depositing User:	Jisc Router
Date Deposited:	16 Sep 2020 17:08
Last Modified:	06 Feb 2024 16:04
URI:	https://repository.uwl.ac.uk/id/eprint/7312