Novel methicillin-resistant Staphylococcus aureus CC8 clone identified in a hospital setting in Armenia

Jamrozy, Dorota, Misra, Raju, Xu, Zhen, Ter-Stepanyan, Mary M., Kocharyan, Karine S., Cave, Rory, Hambardzumyan, Armen Dz and Mkrtchyan, Hermine (2019) Novel methicillin-resistant Staphylococcus aureus CC8 clone identified in a hospital setting in Armenia. Frontiers in Microbiology, 10. ISSN 1664-302X

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Abstract

Whole-genome sequencing (WGS) of methicillin-resistant Staphylococcus aureus (MRSA) has been sparse in low- and middle-income countries, therefore, its population structure is unknown for many regions. We conducted a pilot surveillance of MRSA in the maternity ward of a teaching hospital in Armenia, to characterize the genotypes of circulating MRSA clones. In total, 10 MRSA isolates from a hospital environment (n = 4) and patients (n = 6) were recovered between March and May 2015 and April and May 2016, respectively. WGS analysis showed that the isolates belonged to two clonal complexes (CCs): CC8 (n = 8) and CC30 (n = 2). MRSA CC30 isolates carried staphylococcal cassette chromosome mec (SCCmec) type IVa, whereas MRSA CC8 revealed a type-VT-related SCCmec, which contained a CRISPR/Cas array and showed a high similarity to SCCmec found in coagulase-negative staphylococci. All but one MRSA CC8 isolates carried a plasmid identical to the pSK67 and four also carried a pathogenicity island similar to SaPI5. Phylogenetic analysis showed that the MRSA CC8 isolates formed a monophyletic cluster, which emerged around 1995 and was distinct from representatives of globally-distributed MRSA CC8 lineages. WGS characterization of MRSA in countries with no previous S. aureus genomic surveillance can therefore reveal an unrecognized diversity of MRSA lineages.

Item Type: Article
Additional Information: This is the publisher's final version of the following article: [Jamrozy D, Misra R, Xu Z, Ter-Stepanyan MM, Kocharyan KS, Cave R, Hambardzumyan AD and Mkrtchyan HV (2019) Novel Methicillin-Resistant Staphylococcus aureus CC8 Clone Identified in a Hospital Setting in Armenia. Front. Microbiol. 10:1592. doi: 10.3389/fmicb.2019.01592] first published by Frontiers Media in Frontiers in Microbiology. The final authenticated version is available online at: https://doi.org/10.3389/fmicb.2019.01592 This study was funded by the University of East London internal research grant awarded to HM. DJ was funded by the Wellcome Trust (Grant No. 098051).
Uncontrolled Keywords: methicillin-resistant Staphylococcus aureus, SCCmec, CC8, Papulation genetics, WGS
Subjects: Medicine and health
Related URLs:
Depositing User: Jisc Router
Date Deposited: 11 Sep 2020 15:51
Last Modified: 11 Sep 2020 15:53
URI: http://repository.uwl.ac.uk/id/eprint/7301

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