The mechanism of chlorogenic acid inhibits lipid oxidation: An investigation using multi-spectroscopic methods and molecular docking

Cao, Qiongju, Huang, Yuan, Zhu, Quan-Fei, Song, Mingwei, Xiong, Shanbai, Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722 and Du, Hongying (2020) The mechanism of chlorogenic acid inhibits lipid oxidation: An investigation using multi-spectroscopic methods and molecular docking. Food Chemistry, 333. p. 127528. ISSN 0308-8146

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Cao et al (2020) The mechanism of chlorogenic acid inhibits lipid oxidation - An investigation using multi-spectroscopic methods and molecular docking.pdf - Accepted Version
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Abstract

Endogenous lipase and lipoxygenase play important roles in accelerating lipid oxidation. Polyphenols are a series of commonly used chemicals for preserving fish and seafood products, due to their positive inhibitory effects on lipid oxidation. However, the mechanism involved is still unknown. The inhibitory effects of chlorogenic acid (CGA) on lipase and lipoxygenase were investigated and explored with multi- spectroscopic and molecular docking approaches. Results showed that CGA could inhibit the activities of lipase and lipoxygenase with concentration increased in a highly dose-dependent manner. CGA quenched intrinsic fluorescence intensities of enzymes by static quenching and binding with CGA which led to changes in 3D structures of enzymes. Results of the molecular docking confirmed binding modes, binding sites and major interaction forces between CGA and enzymes, which reduced the corresponding activity. Thus, this study could provide basic mechanisms of the inhibitory effects of polyphenols on lipid oxidation during food preservation.

Item Type: Article
Uncontrolled Keywords: Chlorogenic acid, Lipase, Lipoxygenase, Spectroscopy analysis, Molecular docking
Subjects: Medicine and health > Nutrition
Depositing User: Anne Manyande
Date Deposited: 28 Jul 2020 15:12
Last Modified: 29 Jul 2020 14:12
URI: http://repository.uwl.ac.uk/id/eprint/7212

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