Detection of Acanthamoeba spp. in two major water reservoirs in the Philippines

Milanez, J, Masangkay, F, Hapan, F, Bencito, T, Soriano, j, Ascano, A, Lizarondo, L, Santiago, J, Somsak, Z, Kotepui, M, Tsiami, Amalia ORCID: https://orcid.org/0000-0002-1122-4814, Tangpong, J and Kranis, P (2020) Detection of Acanthamoeba spp. in two major water reservoirs in the Philippines. Journal of Water and Health, 18 (2). pp. 118-126. ISSN 1477-8920

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Abstract

Water reservoirs are important manmade structures providing water security to deliver clean and safe water for drinking and other purposes to the community. Eighty water samples were collected from Magat and Ipo water reservoirs using purposive sampling between November 2018 and January 2019. Water samples were collected in sterile containers for testing. The samples were cultured in nonnutrient agar and lawned with Escherichia coli and incubated at 33 °C. Twelve out of the 80 (15%) water samples were positive for amoebic growth. Light and scanning electron microscopy (SEM) revealed double-walled cystic stages and were initially identified as Acanthamoeba spp. based on morphological characteristic in reference to Page’s established criteria. Their extracted DNAs were used in polymerase chain reaction using JDP1 and JDP2 primers and confirmed the presence of Acanthamoeba DNA in agarose gel electrophoresis. Aligned sequences from PCR products were deposited in GenBank under accession numbers MK886460, MK909919, MK905437, MK910997, MK911021 and MK886514. The presence of potentially pathogenic Acanthamoeba spp. in water reservoirs is considered a potential risk for public health, requiring appropriate processing of water in treatment plants.

Item Type: Article
Uncontrolled Keywords: Acanthamoeba spp., free living amoeba, Ipo reservoir, Magat reservoir, PCR, Philippines
Subjects: Medicine and health > Health promotion and public health > Infection prevention
Medicine and health > Microbiology
Depositing User: Amalia Tsiami
Date Deposited: 28 Apr 2020 17:36
Last Modified: 29 Apr 2020 14:49
URI: http://repository.uwl.ac.uk/id/eprint/6873

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