Regional metabolic patterns of abnormal postoperative behavioral performance in aged mice assessed by 1H-NMR dynamic mapping method

Liu, Taotao, Li, Zhengqian, He, Jindan, Yang, Ning, Han, Dengyang, Li, Yue, Tian, Xuebi, Liu, Huili, Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722, Xiang, Huili, Xu, Fuqiang, Wang, Jie and Guo, Xiangyang (2020) Regional metabolic patterns of abnormal postoperative behavioral performance in aged mice assessed by 1H-NMR dynamic mapping method. Neuroscience Bulletin, 36 (1). pp. 25-38. ISSN 1673-7067

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Abstract

Abstract
Abnormal postoperative neurobehavioral performance (APNP) is a common phenomenon in the early postoperative period. The disturbed homeostatic status of metabolites in the brain after anesthesia and surgery might make a significant contribution to APNP. The dynamic changes of metabolites in different brain regions after anesthesia and surgery, as well as their potential association with APNP are still not well understood. Here, we used a battery of behavioral tests to assess the effects of laparotomy under isoflurane anesthesia in aged mice, and investigated the metabolites in 12 different sub-regions of the brain at different time points using proton nuclear magnetic resonance (1H-NMR) spectroscopy. The abnormal neurobehavioral performance occurred at 6 h and/or 9 h, and recovered at 24 h after anesthesia/surgery. Compared with the control group, the altered metabolite of the model group at 6 h was aspartate (Asp), and the difference was mainly displayed in the cortex; while significant changes at 9 h occurred predominantly in the cortex and hippocampus, and the corresponding metabolites were Asp and glutamate (Glu). All changes returned to baseline at 24 h. The altered metabolic changes could have occurred as a result of the acute APNP, and the metabolites Asp and Glu in the cortex and hippocampus could provide preliminary evidence for understanding the APNP process.

Item Type: Article
Additional Information: This is a post-peer-review, pre-copyedit version of an article published in Neuroscience Bulletin. The final authenticated version is available online at: https://doi.org/10.1007/s12264-019-00414-4
Uncontrolled Keywords: Postoperative abnormal neurobehavioral performance; 1H-NMR; Metabolites; Aspartate; Glutamate
Subjects: Medicine and health > Clinical medicine
Medicine and health
Depositing User: Anne Manyande
Date Deposited: 07 Aug 2019 18:24
Last Modified: 08 Jan 2020 14:06
URI: http://repository.uwl.ac.uk/id/eprint/6313

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