Rabies virus pseudotyped with CVS-N2C glycoprotein as a powerful tool for retrograde neuronal network tracing

Zhu, Xutao, Lin, Kunzhang, Liu, Qing, Yue, Xinpei, Mi, Huijie, Huang, Xiaoping, He, Xiaobin, Wu, Ruiqi, Zheng, Danhao, Wei, Dong, Jia, Liangliang, Wang, Weilin, Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722, Wang, Jie, Zhang, Zhijian and Xu, Fuqiang (2019) Rabies virus pseudotyped with CVS-N2C glycoprotein as a powerful tool for retrograde neuronal network tracing. Neuroscience Bulletin, 36 (3). pp. 202-216. ISSN 1673-7067

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Manyande_etal_NB_2019_Rabies_virus_pseudotyped_with_CVS-N2C_glycoprotein_as_a_powerful_tool_for_retrograde_neuronal_network_tracing_.pdf - Accepted Version

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Abstract

Abstract
Background: Efficient viral vectors for mapping and manipulating long projection neuronal circuits are crucial in brain structural and functional studies. The glycoprotein gene-deleted SAD strain rabies virus pseudotyped with the N2C glycoprotein (SAD-RV(ΔG)-N2C(G)) shows high neuro-tropism in cell culture, but its in vivo retrograde infection efficiency and neuro-tropism have not been systematically characterized.
Methods: SAD-RV(ΔG)-N2C(G) and two other broadly used retrograde tracers, SAD-RV(ΔG)-B19(G) and rAAV2-retro were respectively injected into the VTA or DG in C57BL/6 mice. The neuron numbers labeled across the whole brain regions were counted and analyzed by measuring the retrograde infection efficiencies and tropisms of these viral tools. The labeled neural types were analyzed using fluorescence immunohistochemistry or GAD67-GFP mice.
Result: We found that SAD-RV (ΔG)-N2C (G) enhanced the infection efficiency of long-projecting neurons by ~ 10 times but with very similar neuro-tropism, compared with SAD-RV (ΔG)-B19(G). On the other hand, SAD-RV(ΔG)-N2C(G) showed comparable infection efficiency with rAAV2-retro, but had a more restricted diffusion range, and broader tropism to different types and regions of long-projecting neuronal populations.
Conclusions: These results demonstrate that SAD-RV(ΔG)-N2C(G) can serve as an effective retrograde vector for studying neuronal circuits.
Key words:Viral vector, N2C Glycoprotein, Neuronal circuits, Retrograde tracing

Item Type: Article
Additional Information: This is a post-peer-review, pre-copyedit version of an article published in Neuroscience Bulletin. The final authenticated version is available online at: https://doi.org/10.1007/s12264-019-00423-3
Uncontrolled Keywords: Viral vector, N2C Glycoprotein, Neuronal circuits, Retrograde tracing
Subjects: Medicine and health
Depositing User: Anne Manyande
Date Deposited: 21 Jun 2019 15:18
Last Modified: 22 Aug 2020 02:45
URI: http://repository.uwl.ac.uk/id/eprint/6170

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