Quality of placental RNA: effects of explant size and culture duration

Brew, Obed, Nikolopoulou, Evanthia, Christian, Mark, Sullivan, Mark H.F. and Woodman, Anthony (2016) Quality of placental RNA: effects of explant size and culture duration. Placenta, 46. pp. 45-48. ISSN 0143-4004

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Abstract

We evaluated the impact of placental micro (≤50 mg) and macro (∼200 mg) explants, oxygen concentration and culture method on placental RNA quality after long-term culture. Our findings show that micro explants cultured at 8% oxygen have the best RNA quality and tissue structure. Macro explants were less viable after long-term culture. Macro explants and explants undergoing syncytial degeneration produced poor quality RNA and should be avoided

Item Type: Article
Additional Information: © 2016 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords: Placenta; RNA quality; micro explant; explant viability; oxygen concentration; syncytial regeneration
Subjects: Medicine and health
Depositing User: Obed Brew
Date Deposited: 13 Jun 2016 15:13
Last Modified: 20 Mar 2017 14:29
URI: http://repository.uwl.ac.uk/id/eprint/2539

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