Cavallo, Francesca Romana, Mirza, Khalid, De Mateo, Sara, Rodriguez Manzano, Jesus, Nikolic, Konstantin ORCID: https://orcid.org/0000-0002-6551-2977 and Toumazou, Christofer (2021) A point-of-care device for sensitive protein quantification. In: 2021 IEEE International Symposium on Circuits and Systems (ISCAS), 23-26 May 2021, Daegu, South Korea.

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Abstract

In this paper we present the design of a new point-of-care device for protein quantification. The proposed design is based on a novel aptamer-mediated methodology and real time polymerase chain reaction (RT-PCR), a robust and ultrasensitive method for DNA amplification, which we employ for very sensitive quantification of proteins. In addition, we have also developed an algorithm for the processing of raw fluorescence data from the portable RT-PCR device. The algorithm leads to better linearity than a proprietary software from a commercially available RT-PCR machine. The modular nature of the system allows for easy assembly and adjustment towards a variety of biomarkers for applications in disease diagnosis and personalised medicine.

Item Type:	Conference or Workshop Item (Paper)
ISSN:	2158-1525
ISBN:	9781728192017
Identifier:	10.1109/iscas51556.2021.9401643
Page Range:	pp. 1-5
Identifier:	10.1109/iscas51556.2021.9401643
Additional Information:	© 2021 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
Keywords:	System design, point-of-care, RT-PCR, algorithm, noise minimisation
Subjects:	Construction and engineering > Biomedical engineering
Related URLs:	Publisher
Depositing User:	Konstantin Nikolic
Date Deposited:	15 Jun 2021 12:04
Last Modified:	04 Nov 2024 12:47
URI:	https://repository.uwl.ac.uk/id/eprint/7958

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