Li, L, Xue, X, Zhang, H, Lv, W, Qi, X, Du, H, Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722 and Chen, H (2021) In-situ and one-step preparation of protein film in capillary column for open tubular capillary electrochromatography enantioseparation. Chinese Chemical Letters, 32 (7). pp. 2139-2142. ISSN 1001-8417
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Li et al (2021) In-situ and one-step preparation of protein film in capillary column.pdf - Accepted Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (304kB) | Preview |
Abstract
In this work, the phase-transitioned BSA (PTB) film using the mild and fast fabrication process adhered to the capillary inner wall uniformly and the fabricated PTB film-coated capillary column was applied to realize open tubular capillary electrochromatography (OT-CEC) enantioseparation. The enantioseparation ability of the PTB film-coated capillary was evaluated with eight pairs of chiral analytes including drugs and neurotransmitters, all achieving good resolution and symmetrical peak shape. For three consecutive runs, the relative standard deviations (RSD) of migration time for intra-day, inter-day and column-to-column repeatability were in the range of 0.3-3.5%, 0.2-4.9% and 2.1-7.7%, respectively. Moreover, the PTB film-coated capillary column ran continuously over 300 times with high separation efficiency. Therefore, the coating method based on BSA self-assembly supramolecular film can be extended to the preparation of other proteinaceous capillary columns.
Keywords: Open tubular capillary electrochromatography Proteinaceous phase-transitioned film Bovine serum albumin (BSA) Enantioseparation
In-situ preparation
Item Type: | Article |
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Identifier: | 10.1016/j.cclet.2020.11.022 |
Keywords: | Open tubular capillary electrochromatography, Proteinaceous phase-transitioned film, Bovine serum albumin (BSA), Enantioseparation, In-situ preparation |
Subjects: | Medicine and health > Microbiology Medicine and health > Clinical medicine Medicine and health |
Related URLs: | |
Depositing User: | Anne Manyande |
Date Deposited: | 23 Feb 2021 11:47 |
Last Modified: | 15 Aug 2024 15:00 |
URI: | https://repository.uwl.ac.uk/id/eprint/7686 |
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