Comparative proteomic profiling of methicillin‐susceptible and resistant Staphylococcus aureus

Xu, Zhen, Chen, Jiazhen, Vougas, Kostas, Shah, Ajit, Shah, Haroun, Misra, Raju and Mkrtchyan, Hermine (2020) Comparative proteomic profiling of methicillin‐susceptible and resistant Staphylococcus aureus. Proteomics, 20 (2). p. 1900221. ISSN 1615-9853

Full text not available from this repository.


Staphylococcus aureus is a highly successful human pathogen responsible for a wide range of infections. This study provides insights into the virulence, pathogenicity, and antimicrobial resistance determinants of methicillin‐susceptible and methicillin‐resistant S. aureus (MSSA; MRSA) recovered from non‐healthcare environments. Three environmental MSSA and three environmental MRSA are selected for proteomic profiling using isobaric tag for relative and absolute quantitation tandem mass spectrometry (iTRAQ MS/MS). Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway annotation are applied to interpret the functions of the proteins detected. 792 proteins are identified in MSSA and MRSA. Comparative analysis of MRSA and MSSA reveals that 8 of out 792 proteins are upregulated and 156 are downregulated. Proteins that have differences in abundance are predominantly involved in catalytic and binding activity. Among 164 differently abundant proteins, 29 are involved in pathogenesis, antimicrobial resistance, stress response, mismatch repair, and cell wall synthesis. Twenty‐two proteins associated with pathogenicity including SPA, SBI, CLFA, and DLT are upregulated in MRSA. Moreover, the upregulated pathogenic protein ENTC2 in MSSA is determined to be a super antigen, potentially capable of triggering toxic shock syndrome in the host. Enhanced pathogenicity, antimicrobial resistance, and stress response are observed in MRSA compared to MSSA.

Item Type: Article
Identifier: 10.1002/pmic.201900221
Additional Information: This is the peer reviewed version of the following article: [Xu, Z., Chen, J., Vougas, K., Shah, A., Shah, H., Misra, R., Mkrtchyan, H. V., Comparative Proteomic Profiling of Methicillin‐Susceptible and Resistant Staphylococcus aureus. Proteomics 2020, 20, 1900221. ], which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Keywords: comparative proteomics, methicillin‐resistant Staphylococcus aureus, methicillin‐susceptible Staphylococcus aureus
Subjects: Medicine and health > Health promotion and public health > Infection prevention
Related URLs:
Depositing User: Jisc Router
Date Deposited: 11 Sep 2020 08:44
Last Modified: 06 Feb 2024 16:04

Actions (login required)

View Item View Item