Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb

Zheng, Ning, Wang, Zhi-Zhong, Wang, Song-Wei, Yang, Fang-Jia, Zhu, Xu-Tao, Lu, Chen, Manyande, Anne ORCID:, Rao, Xiao-Ping and Xu, Fu-Qiang (2020) Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb. Zoological Research, 41 (2). pp. 148-156. ISSN 2095-8137

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Manyande_etal_ZooRes_2020_Co-localization_of_two-color_rAAV2-retro_confirms_the_dispersion_characteristics_of_efferent_projections_of_mitral_cells_in_mouse_accessory_olfactory_bulb.pdf - Published Version
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The accessory olfactory bulb (AOB), located at the posterior dorsal aspect of the main olfactory bulb (MOB), is the first brain relay of the accessory olfactory system (AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system (MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by single-color labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis (BST), anterior cortical amygdalar area (ACo), medial amygdaloid nucleus (MeA), and posteromedial cortical amygdaloid area (PMCo) in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB could project to a different number of neurons in different cortices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is encoded and decoded in the AOS.

Item Type: Article
Identifier: 10.24272/j.issn.2095-8137.2020.020
Additional Information: This work was supported by the National Natural Science Foundation of China (31400946, 31771156, 91632303/H09, 91732304 and 31830035) and Strategic Priority Research Program of the Chinese Academy of Sciences (XDB32030200) This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons. org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright ©2020 Editorial Office of Zoological Research, Kunming Institute of Zoology, Chinese Academy of Sciences.
Keywords: Accessory olfactory bulb; Efferent projections; Retrograde rAAVs; Projection neuron labeling; Dispersion characteristics; Circuitry basis
Subjects: Medicine and health
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Depositing User: Users 4141 not found.
Date Deposited: 23 Jan 2020 14:01
Last Modified: 06 Feb 2024 16:01


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