Alterations in amino acid levels and metabolite ratio of spinal cord in rat with myocardial ischemia-reperfusion injury by proton magnetic resonance spectroscopy

Wang, Qian; Li, Zhi-xiao; Li, Yu-juan; Manyande, Anne; Li, Shun-Yuan; Feng, Mao-Hui; Wu, Duo-Zhi; Xiang, Hong-bing

Alterations in amino acid levels and metabolite ratio of spinal cord in rat with myocardial ischemia-reperfusion injury by proton magnetic resonance spectroscopy

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Wang, Qian, Li, Zhi-xiao, Li, Yu-juan, Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722, Li, Shun-Yuan, Feng, Mao-Hui, Wu, Duo-Zhi and Xiang, Hong-bing (2019) Alterations in amino acid levels and metabolite ratio of spinal cord in rat with myocardial ischemia-reperfusion injury by proton magnetic resonance spectroscopy. American Journal of Translational Research, 11 (5). pp. 3101-3108.

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Official URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC65566...

Abstract

Abstract
Objectives The mechanism behind spinal metabolites and myocardial ischemia-reperfusion (IR) injury is not well understood. Proton magnetic resonance spectroscopic analysis of spinal cord extracts provides a quick evaluation of the specific metabolic activity in rats with myocardial IR injury. We investigated the relationship between the IR-related variables and the changes in spinal metabolites.
Methods Proton magnetic resonance spectroscopy (1H-MRS) was used to assess the spinal metabolites of adult rats with and without myocardial IR injury (n=6 per group). Myocardial IR injury was reproduced using intermittent occlusion of the left anterior descending coronary artery. We studied the relationship between the metabolite ratio measurement and IR-related variables. All rats underwent 1H-MRS, with the ratio of interest placed in different spinal cord segments to measure levels of twelve metabolites including N-acetylaspartate (NAA), taurine (Tau),glutamate (Glu),gamma amino acid butyric acid (GABA),creatine (Cr), and myoinositol (MI), etc.
Results Rats with myocardial IR injury had higher concentration of Tau in the upper thoracic spinal cord (p< 0.05), and lower concentration of Gly and Glu in the cervical segment of the spinal cord (p< 0.05), when compared to the Control group. The ratios of glutamate/taurine (Glu/Tau), Glu/(GABA+Tau) and Glu/Total were significantly different between the IR group and the Control group in the upper thoracic spinal cord (p< 0.05). So were the ratios of Glu/(GABA+Tau) in the cervical segment (p< 0.05),and Glu/Tau and Glu/(GABA+Tau) in the lower thoracic spinal cord (p< 0.05).
Conclusions These findings suggest that myocardial IR injury may be related to spinal biochemical alterations. It is speculated that these observed changes in the levels of spinal metabolites may be involved in the pathogenesis and regulation of myocardial IR injury.

Keywords: myocardial ischemia-reperfusion injury; spinal cord; metabolomics; proton nuclear magnetic resonance

Item Type:	Article
Keywords:	myocardial ischemia-reperfusion injury; spinal cord; metabolomics; proton nuclear magnetic resonance
Subjects:	Medicine and health > Clinical medicine Medicine and health Medicine and health > Physiology
Related URLs:	Publisher Publisher
Date Deposited:	17 May 2019
Dates:	Date Publication status 15 May 2019 Published Online 1 March 2019 Accepted
School, department or research centre:	School of Human and Social Sciences
Keywords:	myocardial ischemia-reperfusion injury; spinal cord; metabolomics; proton nuclear magnetic resonance
URI:	https://repository.uwl.ac.uk/id/eprint/6095

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