Immunization with bacillus Calmette-Guerin vaccine increases aortic atherosclerosis in the cholesterol-fed rabbit

Lamb, D. J., Eales-Reynolds, Lesley-Jane ORCID: 0000-0002-9906-2484 and Ferns, G. A. (1999) Immunization with bacillus Calmette-Guerin vaccine increases aortic atherosclerosis in the cholesterol-fed rabbit. Atherosclerosis, 143 (1). pp. 105-113. ISSN 0021-9150

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Abstract

New Zealand White rabbits were injected subcutaneously with either a human dose of bacillus Calmette Guerin (BCG) vaccine (n = 7) or saline (n = 7). A further half dose of BCG or saline was injected after a further 4 weeks. The animals were subsequently fed a 0.25-1% cholesterol diet for 10 weeks, 8 weeks after the first injection. The rabbits were killed and perfusion fixed with 4% paraformaldehyde. The integrated plasma cholesterol levels did not differ significantly between the groups (P \textgreater 0.05). Plasma levels of anti-mycobacterial antibodies rose following BCG immunization, reaching a peak after 8 weeks (P \textless 0.05) compared to basal titers and the control group. BCG immunization was also associated with increased peripheral lymphocyte and monocyte activation, as evidenced by increased surface expression of IL-2 receptor (CD25) (P \textless 0.02) and MAC-I (CD11b) (P \textless 0.05), respectively. Significantly more mononuclear cells bound to the aortic endothelium of BCG immunized, cholesterol-fed rabbits (1.93+/-0.77 mononuclear cells/1000 endothelial cells) than to that of saline immunized rabbits (0.08+/-0.08 mononuclear cells/1000 endothelial cells; P \textless 0.01). The aortic intimal:medial ratio was greater in the BCG immunized rabbits (0.19+/-0.08) than those treated with saline (0.04+/-0.03; P \textless 0.05). This suggests that BCG immunization enhances peripheral leucocyte activation, aortic monocyte recruitment and atherogenesis in the cholesterol-fed rabbit.

Item Type: Article
Uncontrolled Keywords: Animals, Lymphocyte Activation, Monocytes, Antibodies, Bacterial, Antigens, CD11, Aorta, Aortic Diseases, Arteriosclerosis, BCG Vaccine, Cell Adhesion, Cholesterol, Cholesterol, Dietary, Immunization, Leukocytes, Mononuclear, Mycobacterium, Rabbits, Receptors, Interleukin-2
Subjects: Medicine and health > Microbiology
Medicine and health
Depositing User: Lesley-Jane Eales-Reynolds
Date Deposited: 20 May 2018 08:09
Last Modified: 22 May 2018 09:45
URI: http://repository.uwl.ac.uk/id/eprint/5056

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