Basheer, Haneen A, Elsalem, Lina, Salem, Anwar, Tailor, Artysha, Hunter, Keith and Afarinkia, Kamyar ORCID: https://orcid.org/0000-0003-2819-4364 (2022) The Expression of Glutaminases and their association with clinicopathological parameters in the head and neck cancers. Current Cancer Drug Targets, 22 (2). pp. 169-179. ISSN 1568-0096

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Abstract

Background: The increased glutamine metabolism is a characteristic feature of cancer cells. The interconversion between glutamine and glutamate is catalyzed by two glutaminase isoforms, GLS1 and GLS2, which appear to have different roles in different types of cancer. We investigated for the first time the protein expression of GLS1 and GLS2, and their correlation with advanced clinicopathological parameters in head and neck cancers.

Methods: Consecutive slides from a tissue microarray comprised of 80 samples ranging from normal to metastatic were stained immunohistochemically for GLS1, GLS2, HIF-1α or CD147. Following analysis by two expert pathologists, we carried out a statistical analysis of the scores.

Results: GLS1 and GLS2 were found to be upregulated at the protein level in head and neck tumours compared to normal tissues, and this increased expression correlated positively (GLS1) and negatively (GLS2) with tumor grade, indicating a shift of expression between GLS enzyme isoforms based on tumor differentiation. Increased expression of GLS1 was associated with high CD147 expression, and elevated GLS2 expression was associated with both high CD147 and high HIF-1α expressions. The correlation of the GLS1 and GLS2 with HIF-1α or CD147 was strongly associated with more advanced clinicopathological parameters.

Conclusion: The increased expression of GLS1 and GLS2 may be explored as a new treatment for head and neck cancers.

Item Type:	Article
Identifier:	10.2174/1568009622666211224111425
Keywords:	Glutaminases, GLS1, GLS2, CD147, immunohistochemistry, head and neck cancers
Subjects:	Medicine and health > Clinical medicine
Date Deposited:	07 Sep 2026
Dates:	Date Publication status 28 October 2021 Accepted 3 March 2022 Published
School, department or research centre:	School of Medicine and Biosciences
Keywords:	Glutaminases, GLS1, GLS2, CD147, immunohistochemistry, head and neck cancers
URI:	https://repository.uwl.ac.uk/id/eprint/15292

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