Moradian, Shafagh, Ali, Nida, Jagadeesan, Rahul, Behrends, Volker ORCID: https://orcid.org/0000-0003-4855-5497, Sanches Ribeiro, Andre and Engl, Christoph (2026) Premature transcription termination modulates stochastic gene expression in bacteria. Science Advances, 15 (20).

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Abstract

Bacteria regulate transcription by controlling its initiation and prematurely terminating it through attenuation. Transcriptional regulation produces RNA bursts defined by size and frequency. While bursting via regulated initiation is well characterized, how attenuation shapes burst dynamics remains poorly understood. We show that in Escherichia coli, attenuation combined with regulated initiation in the tryptophan operon (trp) modulates burst size and frequency, producing switch-like transitions in transcriptional activity between the tryptophan presence and absence. In Bacillus subtilis, lacking regulated trp initiation, attenuation modulates burst size, gradually changing transcriptional activity as the tryptophan concentration increases. These distinct architectures may reflect adaptive strategies: B. subtilis maintains highly expressing cells over a wider tryptophan range, likely beneficial in soil where tryptophan is scarce and unevenly distributed, while E. coli enables rapid repression suited to fluctuating gut environments. Notably, trp transcription is not strictly cell-autonomous. Highly expressing cells can suppress transcription in neighboring cells, revealing intercellular regulation and supporting community-level cooperation.

Item Type:	Article
Identifier:	10.1126/sciadv.aed0831
Subjects:	Medicine and health > Microbiology Natural sciences > Cell and molecular biology
Date Deposited:	16 Jun 2026
Dates:	Date Publication status 2 April 2026 Accepted 15 May 2026 Published
School, department or research centre:	School of Medicine and Biosciences
URI:	https://repository.uwl.ac.uk/id/eprint/15051

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