Morgan, Richard ORCID: https://orcid.org/0000-0002-8721-4479, Smith, Christopher and Pandha, Hardev (2025) Using the conserved hexapeptide in HOX proteins as an antagonist of HOX/PBX interactions. In: HOX Genes: Methods and Protocols. Methods in Molecular Biology (2889). Springer Nature, New York, USA. ISBN 9781071643228

PDF (12 month embargo) Targeting HOX-PBX interactions accepted version for UWL rep.pdf - Accepted Version Restricted to Repository staff only until 3 January 2026. Download (523kB)

Abstract

The HOX and PBX genes encode transcription factors that have key roles in development and cancer, both independently and as a heterodimer within a complex of proteins that recognizes specific sequences in DNA and can both activate or repress transcription of target genes. Due to functional redundancy amongst HOX proteins, knock down or knock out studies of individual genes often do not result in an altered phenotype. An alternative approach is to target the interaction between HOX and PBX proteins, which is dependent on a conserved hexapeptide region within HOX. To this end, several peptides have been developed based on the hexapeptide sequence which act as competitive antagonists of HOX/PBX binding, including HXR9 and HTL001. Here, we review the methodology that has been used in these studies, including peptide syntheses, cell culture, assays, and mouse models.

Item Type:	Book Section
Identifier:	10.1007/978-1-0716-4322-8_10
Additional Information:	Permission to view, print, copy, download and text and data-mine the content, for the purposes of academic research. https://www.springernature.com/gp/open-science/policies/journal-policies and https://www.springernature.com/gp/open-science/policies/accepted-manuscript-terms
Subjects:	Natural sciences > Cell and molecular biology
Related URLs:	https://link.springer.com/book/10.1007/9...
Depositing User:	Richard Morgan
Date Deposited:	17 Jan 2025 06:16
Last Modified:	17 Jan 2025 08:00
URI:	https://repository.uwl.ac.uk/id/eprint/13098

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