Taylor, Rachel, Swift, Thomas, Wilkinson, David and Afarinkia, Kamyar ORCID: https://orcid.org/0000-0003-2819-4364 (2024) A method for estimation of plasma protein binding using diffusion ordered NMR spectroscopy (DOSY). RSC Medicinal Chemistry.

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Abstract

The plasma protein binding (PPB) of a drug plays a key role in both its pharmacokinetic and
pharmacodynamic properties. During lead optimisation, medium and high throughput methods for the early determination of PPB can provide important information about potential PKPD profile within a chemotype or between different chemotype series. Diffusion ordered spectroscopy (DOSY) is an NMR spectroscopic technique that measures the diffusion of a molecule through the magnetic field gradient, according to its molecular size/weight. Here, we describe the use of DOSY for a rapid and straight forward method to evaluate the PPB of drug molecules, using their binding to bovine serum albumin (BSA) as a model.

Item Type:	Article
Identifier:	10.1039/D4MD00244J
Subjects:	Natural sciences > Cell and molecular biology Natural sciences
Depositing User:	Kamyar Afarinkia
Date Deposited:	01 Jul 2024 14:18
Last Modified:	04 Nov 2024 11:03
URI:	https://repository.uwl.ac.uk/id/eprint/12066

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