Katzarov, Stoyko and Behrends, Volker ORCID: https://orcid.org/0000-0003-4855-5497 (2022) Temporal Hierarchy and Context-Dependence of Quorum Sensing Signal in Pseudomonas aeruginosa. Life, 12 (12). p. 1953.

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Abstract

The Gram-negative bacterium Pseudomonas aeruginosa can cause infections in a broad range of hosts including plants, invertebrates and mammals and is an important source of nosocomial infections in humans. We were interested in how differences in the bacteria’s nutritional environment impact bacterial communication and virulence factor production. We grew P. aeruginosa in 96 different conditions in BIOLOG Gen III plates and assayed quorum sensing (QS) signaling over the course of growth. We also quantified pyocyanin and biofilm production and the impact of sub-inhibitory exposure to tobramycin. We found that while 3-oxo-C12 homoserine lactone remained the dominant QS signal to be produced, timing of PQS production differed between media types. Further, whether cells grew predominantly as biofilms or planktonic cells was highly context dependent. Our data suggest that understanding the impact of the nutritional environment on the bacterium can lead to valuable insights into the link between bacterial physiology and pathology.

Item Type:	Article
Identifier:	10.3390/life12121953
Subjects:	Medicine and health
Depositing User:	Marc Forster
Date Deposited:	12 Apr 2024 07:53
Last Modified:	12 Apr 2024 07:54
URI:	https://repository.uwl.ac.uk/id/eprint/11489

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