Bravo-Santano, Natalia, Ellis, James K, Calle, Yolanda, Keun, Hector C, Behrends, Volker ORCID: https://orcid.org/0000-0003-4855-5497 and Letek, Michal (2019) Intracellular Staphylococcus aureus elicits the production of host very long-chain saturated fatty acids with antimicrobial activity. Metabolites, 9 (7). p. 148.

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Abstract

As a facultative intracellular pathogen, Staphylococcus aureus is able to invade and proliferate within many types of mammalian cells. Intracellular bacterial replication relies on host nutrient supplies and, therefore, cell metabolism is closely bound to intracellular infection. Here, we investigated how S. aureus invasion affects the host membrane-bound fatty acids. We quantified the relative levels of fatty acids and their labelling pattern after intracellular infection by gas chromatography-mass spectrometry (GC-MS). Interestingly, we observed that the levels of three host fatty acids—docosanoic, eicosanoic and palmitic acids—were significantly increased in response to intracellular S. aureus infection. Accordingly, labelling carbon distribution was also affected in infected cells, in comparison to the uninfected control. In addition, treatment of HeLa cells with these three fatty acids showed a cytoprotective role by directly reducing S. aureus growth.

Item Type:	Article
Identifier:	10.3390/metabo9070148
Subjects:	Medicine and health
Depositing User:	Marc Forster
Date Deposited:	12 Apr 2024 08:58
Last Modified:	12 Apr 2024 08:58
URI:	https://repository.uwl.ac.uk/id/eprint/11477

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