Xu, Zeru, Hu, Hongying, Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722 and Xiong, Shanbai (2023) A comprehensive investigation on the interaction between jaceosidin, baicalein and lipoxygenase: Multi-spectroscopic analysis and computational study. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 304.

Preview

PDF Xu et al (2024) A comprehensive investigation on the interaction between jaceosidin, baicalein and lipoxygenase_Multi-spectroscopic analysis and computational study.pdf - Published Version Available under License Creative Commons Attribution. Download (7MB) | Preview

Abstract

Lipoxygenase (LOX) has the harmful effect of accelerating lipid oxidation, and polyphenols have the inhibitory effect on lipoxygenase. However, there were rare researches investigated on the interactions between polyphenols and LOX. In this study, the binding mechanisms between polyphenols (Jaceosidin-JSD and baicalein-BCL) and LOX were investigated by multi-spectroscopic analysis and computational study. Both JSD and BCL binding to LOX resulted in static fluorescence quenching, and the complexes of JSD-LOX and BCL-LOX were built at a molar ratio of 1:1, respectively. The binding constants of LOX-JSD (72.18 × 105 L/mol at 298 K) and LOX-BCL (12.43 × 105 L/mol at 298 K) indicated that LOX had stronger binding affinity to JSD compared to BCL. Compared with BCL-LOX, the JSD-LOX system formed more hydrogen bonds which ensured a stronger bond between JSD and LOX. The studies in molecular dynamics also demonstrated that the JSD-LOX complex is more stable, and the addition of JSD is more conducive to the complex formation. The current study provides some new insights for the study on the inhibition of lipid oxidation and affords a new strategy for the discovery of novel food preservatives.

Item Type:	Article
Identifier:	10.1016/j.saa.2023.123423
Subjects:	Medicine and health
Depositing User:	Marc Forster
Date Deposited:	11 Apr 2024 08:58
Last Modified:	11 Apr 2024 08:58
URI:	https://repository.uwl.ac.uk/id/eprint/11435

Downloads

Actions (login required)

View Item