La Rosa, Ruggero, Behrends, Volker ORCID: https://orcid.org/0000-0003-4855-5497, Williams, Huw D., Bundy, Jacob G. and Rojo, Fernando (2016) Influence of the Crc regulator on the hierarchical use of carbon sources from a complete medium in Pseudomonas. Environmental Microbiology, 18 (3). pp. 807-818. ISSN 1462-2912

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Abstract

The Crc protein, together with the Hfq protein, participates in catabolite repression in pseudomonads,
helping to coordinate metabolism. Little is known about how Crc affects the hierarchy of metabolite
assimilation from complex mixtures. Using proton Nuclear Magnetic Resonance (NMR) spectroscopy, we
carried out comprehensive metabolite profiling of culture supernatants (metabolic footprinting)
over the course of growth of both Pseudomonas putida and P. aeruginosa, and compared the wild-type strains
with deletion mutants for crc. A complex metabolite consumption hierarchy was observed, which was
broadly similar between the two species, although with some important differences, for example in sugar
utilization. The order of metabolite utilization changed upon inactivation of the crc gene, but even in the Crc-null strains
some compounds were completely consumed before late metabolites were taken up.
This suggests the presence of additional regulatory elements that determine the time and order of consumption of compounds.
Unexpectedly, the loss of Crc led both species to excrete acetate and pyruvate as a result of unbalanced growth during exponential phase,
compounds that were later consumed in stationary phase.
This loss of carbon during growth helps to explain the contribution of the Crc/Hfq regulatory system to evolutionary fitness of pseudomonads.

Item Type:	Article
Identifier:	10.1111/1462-2920.13126
Subjects:	Natural sciences > Cell and molecular biology Natural sciences
Depositing User:	Volker Behrends
Date Deposited:	29 Apr 2024 09:59
Last Modified:	13 Jan 2025 15:19
URI:	https://repository.uwl.ac.uk/id/eprint/11395

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