Bioengineering of antibody fragments: challenges and opportunities

Pirkalkhoran, Sama, Grabowska, Wiktoria Roksana, Kashkoli, Hamid Heidari, Mirhassani, Reihaneh, Guiliano, David, Dolphin, Colin and Khalili, Hanieh ORCID: (2023) Bioengineering of antibody fragments: challenges and opportunities. Bioengineering, 10 (2). p. 122.

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Antibody fragments are used in the clinic as important therapeutic proteins for treatment of indications where better tissue penetration and less immunogenic molecules are needed. Several expression platforms have been employed for the production of these recombinant proteins, from which E. coli and CHO cell-based systems have emerged as the most promising hosts for higher expression. Because antibody fragments such as Fabs and scFvs are smaller than traditional antibody structures and do not require specific patterns of glycosylation decoration for therapeutic efficacy, it is possible to express them in systems with reduced post-translational modification capacity and high expression yield, for example, in plant and insect cell-based systems. In this review, we describe different bioengineering technologies along with their opportunities and difficulties to manufacture antibody fragments with consideration of stability, efficacy and safety for humans. There is still potential for a new production technology with a view of being simple, fast and cost-effective while maintaining the stability and efficacy of biotherapeutic fragments.

Item Type: Article
Identifier: 10.3390/bioengineering10020122
Keywords: bioengineering; biotherapeutics; antibody fragments; baculovirus expression system; plants expression system
Subjects: Construction and engineering > Biomedical engineering
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Depositing User: Hanieh Khalili
Date Deposited: 20 Apr 2023 15:18
Last Modified: 06 Feb 2024 16:14


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