Intralesional infiltrations of arteriosclerotic tissue cells-free filtrate reproduce vascular pathology in healthy recipient rats

Berlanga-Acosta, Jorge, Fernández-Mayola, Maday, Mendoza-Marí, Yssel, García-Ojalvo, Ariana, Martinez-Jimenez, Indira, Rodriguez-Rodriguez, Nadia, Playford, Raymond J. ORCID:, Reyes-Acosta, Osvaldo, Lopez-Marín, Laura and Guillén-Nieto, Gerardo (2022) Intralesional infiltrations of arteriosclerotic tissue cells-free filtrate reproduce vascular pathology in healthy recipient rats. International Journal of Molecular Sciences, 23 (3). p. 1511. ISSN 1661-6596

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Berlanga-Acosta_et_al._(2021)_ijms._Intralesional_Infiltrations_of_Arteriosclerotic_Tissue_Cells-Free_Filtrate_Reproduce_Vascular_Pathology_in_Healthy_Recipient_Rats.pdf - Published Version
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Lower-extremity arterial disease is a major health problem with increasing prevalence, often leading to non-traumatic amputation, disability and mortality. The molecular mechanisms underpinning abnormal vascular wall remodeling are not fully understood. We hypothesized on the existence of a vascular tissue memory that may be transmitted through soluble signaling messengers, transferred from humans to healthy recipient animals, and consequently drive the recapitulation of arterial wall thickening and other vascular pathologies. We examined the effects of the intralesional infiltration for 6 days of arteriosclerotic popliteal artery-derived homogenates (100 µg of protein) into rats’ full-thickness wounds granulation tissue. Animals infiltrated with normal saline solution or healthy brachial arterial tissue homogenate obtained from traumatic amputation served as controls. The significant thickening of arteriolar walls was the constant outcome in two independent experiments for animals receiving arteriosclerotic tissue homogenates. This material induced other vascular morphological changes including an endothelial cell phenotypic reprogramming that mirrored the donor’s vascular histopathology. The immunohistochemical expression pattern of relevant vascular markers appeared to match between the human tissue and the corresponding recipient rats. These changes occurred within days of administration, and with no cross-species limitation. The identification of these “vascular disease drivers” may pave novel research avenues for atherosclerosis pathobiology.

Item Type: Article
Identifier: 10.3390/ijms23031511
Additional Information: Citation: Berlanga-Acosta, J.; Fernández-Mayola, M.; Mendoza-Marí, Y.; García-Ojalvo, A.; Martinez-Jimenez, I.; Rodriguez-Rodriguez, N.; Playford, R.J.; Reyes-Acosta, O.; Lopez-Marín, L.; Guillén-Nieto, G. Intralesional Infiltrations of Arteriosclerotic Tissue Cells-Free Filtrate Reproduce Vascular Pathology in Healthy Recipient Rats. Int. J. Mol. Sci. 2022, 23, 1511. 10.3390/ijms23031511 © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
Keywords: critical limb ischemia; arterial disease; arteriosclerosis; angiopathy
Subjects: Natural sciences > Cell and molecular biology
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Depositing User: Raymond J. Playford
Date Deposited: 15 Mar 2022 11:43
Last Modified: 06 Feb 2024 16:09


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