NMR based metabolomics comparison of different blood sampling techniques in awake and anesthetized rats

Du, Hongying, Li, Shuang, Zhang, Yingfeng, Guo, Huiling, Wu, Liang, Liu, Huili, Manyande, Anne ORCID: https://orcid.org/0000-0002-8257-0722, Xu, Fuqiang and Wang, Jie (2019) NMR based metabolomics comparison of different blood sampling techniques in awake and anesthetized rats. Molecules, 24 (14). p. 2542.

[thumbnail of Manyande_etal_Molecules_2019_NMR_based_metabolomics_comparison_of_different_blood_sampling_techniques_in_awake_and_anesthetized_rats.pdf]
Manyande_etal_Molecules_2019_NMR_based_metabolomics_comparison_of_different_blood_sampling_techniques_in_awake_and_anesthetized_rats.pdf - Published Version
Available under License Creative Commons Attribution.

Download (11MB) | Preview


The composition of body fluids has become one of the most commonly used methods for diagnosing various diseases or monitoring the drug responses, especially in serum/plasma. It is therefore vital for investigators to find an appropriate way to collect blood samples from laboratory animals. This study compared blood samples collected from different sites using the NMR based metabolomics approach. Blood samples were collected from the saphenous vein (awake state), tail vein (awake and anesthetized states after administration of sevoflurane or pentobarbital) and the inferior thoracic vena cava (ITVC, anesthetized state). These approaches from the saphenous and tail veins have the potential to enable the collection of multiple samples, and the approach from ITVC is the best method for the collection of blood for the terminate state. The compositions of small molecules in the serum were determined using the 1H-NMR method, and the data were analyzed with traditional correlation analysis, principle component analysis (PCA) and OPLS-DA methods. The results showed that acute anesthesia significantly influenced the composition of serum in a very short period, such as the significant increase in glucose, and decrease in lactate. This indicates that it is better to obtain blood samples under the awake state. From the perspective of animal welfare and multiple sampling, the current study shows that the saphenous vein and tail vein are the best locations to collect multiple blood samples for a reduced risk of injury in the awake state. Furthermore, it is also suitable for investigating pharmacokinetics and the effects of drug intervention on animals.

Item Type: Article
Identifier: 10.3390/molecules24142542
Additional Information: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Keywords: blood collection; serum; NMR; metabolomics; saphenous vein
Subjects: Psychology
Depositing User: Users 4141 not found.
Date Deposited: 17 Jul 2019 14:27
Last Modified: 06 Feb 2024 16:00
URI: https://repository.uwl.ac.uk/id/eprint/6273


Downloads per month over past year

Actions (login required)

View Item View Item